The International Society for Stem Cell Research (ISSCR) - https://www.isscr.org/ - recently hosted a standards webinar. Among other things, the webinar discussions highlighted the need for multiple independent clones when comparing isogenic cells derived from genome manipulation. With iPSCs it is difficult to: (a) obtain and screen multiple clones; and (b) culture, expand and assess
Okay – a cliché – but it is said that a picture is worth 1,000 words. And we continue to believe that pictures of iPSCs can be revealing. Here at Cellected we have been carrying out some pain-staking and detailed research around the different outcomes you can get by optimising (or not) your media, supplements,
One of the team here at Cellected produced an elegant Christmas tree using extracts from immunocytochemistry stains that she had been working on over the last few months. We then thought that for those not fluent in iPSC pluripotency assays, it might be helpful to add a small festive explanation - hence the festive-infographic below.
Are you having difficulty single cell cloning your iPSC’s - or just find the process frustrating, time consuming and/or not a great use of your team's time? Do you have poor growth or low efficiency/multiple gene edits - and need to screen high numbers of clones? Can Cellected help you? Cellected's single cell cloning technology
The images generated by immunocytochemistry (ICC) and immunofluorescence (IF) in relation to iPSC pluripotency marker panels can be stunning. You might say more art than science… The team here at Cellected has been working hard at optimising the ICC/IF processes with a variety of plate formats and thought that we should share some of the
Genomic stability assessment is a critical part of iPSC QC but unfortunately a number of methods, whilst highly informative, can give rise to a long wait for results. This means that the genomic stability data are often obtained after the iPSCs have been banked / cryopreserved. We are building in-process QC, to complement the traditional
We thought that you might be interested in a quick overview of an iPSC "rescue mission". Or, put another way, an example of some of the challenges of working with iPSCs and traditional protocols / media. All iPSC scientists will be familiar with this situation: we revived some cells with their recommended media and protocols;
We spent the summer working hard on our new website. (Confession: we have a huge number of 'gorgeous' images of stem cells - so the usual challenge about getting decent photos of iPSCs is not a problem chez Cellected.) We will update you with new developments at Cellected as the business - and our client