Okay – a cliché – but it is said that a picture is worth 1,000 words. And we continue to believe that pictures of iPSCs can be revealing.
Here at Cellected we have been carrying out some pain-staking and detailed research around the different outcomes you can get by optimising (or not) your media, supplements, matrix and reagents to suit your cells and processes. Although it is time-consuming, such optimisation work is incredibly important when you need to consider, for example, protocols that require the dissociation of iPS cells into a single cell suspension
The images above are all derived from the same cell line and demonstrate the range of morphologies for colonies formed 6 days after seeding as a single cell suspension into 96-well plates with a range of conditions. Morphology, of course, is only one assessment for the cells!
If media and reagent testing is – or you just have a suspicion that it may be – hindering your process, slowing you down or preventing you performing successfully key R&D, then do get in touch as we will be able to help you find a robust and optimal solution!
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