Cellected is a specialist service business, focusing on induced Pluripotent Stem Cells (iPSCs)
Cellected is a specialist service business, focusing on induced Pluripotent Stem Cells (iPSCs)
We have extensive expertise in iPSCs – especially clonal cell line derivation, process and protocol development, QC, characterisation and banking.
We also have a semi-automated platform for clonal cell line derivation that allows the generation of high numbers of clonal cell lines which enables the efficient generation of datasets for regulatory submissions.
Clonal cell line derivation
Many processes require clonal cell lines including re-programming and gene-editing. Hence it is important to be certain that your iPSC cell line is originated from a single cell.
Do you have robust data – that will withstand regulatory scrutiny – to support mono-clonality?
Cellected has invested in state-of-the-art single cell seeding technology that supports the establishment of monoclonal iPSC lines.
Stem cell banking, QC & characterisation
iPSCs can be challenging to work with. They can readily lose their “stemness”. Ensuring their ongoing undifferentiated state and genomic stability is critical.
Cellected can help with the ‘QC’ and characterisation of your iPSCs.
Validation of new reagents & technologies for iPSCs
We have the experience and the facilities to carry out detailed validation studies for new technologies and reagents associated with iPSCs.
In consultation with your technical teams, we can prepare a detailed and highly bespoke package of work.
Why Choose Our Services?
Our expertise and streamlined approach ensures we can support your complex R&D projects with our complementary QC and characterisation services.
Our platform is built with throughput and efficiency in mind, helping us to help you meet your deadlines.
Each project is designed in consultation with the client, ensuring it is bespoke and tailored to your specific needs.
Our Team
Dr Claire Richards and Dr Rosa Loffredo lead Cellected’s team of scientist and technicians. Claire and Rosa are highly experienced cell biologists with extensive and complementary experience working with iPSCs (and other cell lines).
EXPLOITING NEW TECHNOLOGIES
Our core services have been built around the use of new technology, including automation, novel reagents and latest generation microscopy
Our platform combines the latest technologies in single cell cloning and imaging with gold standard traditional QC approaches such as immunocytochemistry, immunofluoresence, digital PCR, microarray-based SNP analysis and G-band karyotyping to carry out comprehensive characterisation of iPSCs.
The combination of these assays and new technologies enables Cellected to provide clients with a high throughput platform focussed on state-of-the-art quality control.
Consulting services for iPSCs from Cellected
Drawing upon its expertise in iPSCs, Cellected is able to provide clients with a range of complementary consulting services – which are being added to, and expended, all the time.
If you have a particular service that you would like us to provide – but is not on the list below – then please contact us: it is most likely that Cellected will be able to help you.
How best to QC and characterise your iPSCs
Validation of new iPSC lines
Advice re. handling of iPSCs and protocol development
Training in relation to iPSCs and/or cell culture
Trouble-shooting
Laboratory set-up, construction and equipment specification
Instrument, technology and reagent validation
The International Society for Stem Cell Research (ISSCR) – https://www.isscr.org/ – recently hosted a standards webinar. Among other things, the webinar discussions highlighted the need for multiple independent clones when comparing isogenic cells derived from genome manipulation. With iPSCs it is difficult to: (a) obtain and screen multiple clones; and (b) culture, expand and assess […]
Okay – a cliché – but it is said that a picture is worth 1,000 words. And we continue to believe that pictures of iPSCs can be revealing. Here at Cellected we have been carrying out some pain-staking and detailed research around the different outcomes you can get by optimising (or not) your media, supplements, […]
One of the team here at Cellected produced an elegant Christmas tree using extracts from immunocytochemistry stains that she had been working on over the last few months. We then thought that for those not fluent in iPSC pluripotency assays, it might be helpful to add a small festive explanation – hence the festive-infographic below. […]
