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The critical importance of obtaining multiple independent clones

2023-01-27T11:45:03+00:00January 27th, 2023|Quick update|

The International Society for Stem Cell Research (ISSCR) - https://www.isscr.org/  - recently hosted a standards webinar.  Among other things, the webinar discussions highlighted the need for multiple independent clones when comparing isogenic cells derived from genome manipulation. With iPSCs it is difficult to: (a) obtain and screen multiple clones; and (b) culture, expand and assess

The importance of optimising your reagents, media, supplements and matrix

2023-01-23T15:42:47+00:00January 23rd, 2023|Quick update|

Okay – a cliché – but it is said that a picture is worth 1,000 words. And we continue to believe that pictures of iPSCs can be revealing. Here at Cellected we have been carrying out some pain-staking and detailed research around the different outcomes you can get by optimising (or not) your media, supplements,

The festive pluripotency infographic from Cellected – built around a Christmas tree

2022-12-16T14:31:56+00:00December 16th, 2022|Quick update|

One of the team here at Cellected produced an elegant Christmas tree using extracts from immunocytochemistry stains that she had been working on over the last few months. We then thought that for those not fluent in iPSC pluripotency assays, it might be helpful to add a small festive explanation - hence the festive-infographic below.

Single cell cloning of iPSCs at Cellected

2022-11-25T13:41:53+00:00November 25th, 2022|Quick update|

Are you having difficulty single cell cloning your iPSC’s - or just find the process frustrating, time consuming and/or not a great use of your team's time? Do you have poor growth or low efficiency/multiple gene edits - and need to screen high numbers of clones? Can Cellected help you? Cellected's single cell cloning technology

Optimising ICC/IF for iPSCs

2022-10-18T10:28:37+01:00October 18th, 2022|Quick update|

The images generated by immunocytochemistry (ICC) and immunofluorescence (IF) in relation to iPSC pluripotency marker panels can be stunning. You might say more art than science… The team here at Cellected has been working hard at optimising the ICC/IF processes with a variety of plate formats and thought that we should share some of the

Use of ddPCR to monitor genomic aberration hotspots in iPSCs

2022-10-17T13:04:23+01:00October 17th, 2022|Quick update|

Genomic stability assessment is a critical part of iPSC QC but unfortunately a number of methods, whilst highly informative, can give rise to a long wait for results.  This means that the genomic stability data are often obtained after the iPSCs have been banked / cryopreserved. We are building in-process QC, to complement the traditional

iPSC ‘Rescue Mission’: optimised media and reagents can have a big impact on iPSC colony health

2022-09-23T11:45:18+01:00September 14th, 2022|Quick update|

We thought that you might be interested in a quick overview of an iPSC "rescue mission". Or, put another way, an example of some of the challenges of working with iPSCs and traditional protocols / media. All iPSC scientists will be familiar with this situation: we revived some cells with their recommended media and protocols;

Cellected launches its new website

2022-09-14T11:03:54+01:00September 7th, 2022|Quick update|

We spent the summer working hard on our new website. (Confession: we have a huge number of 'gorgeous' images of stem cells - so the usual challenge about getting decent photos of iPSCs is not a problem chez Cellected.) We will update you with new developments at Cellected as the business - and our client

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