iPSC clonal cell line generation
For many workflows, especially those requiring isogenic and gene edited cell lines, it is essential to generate a truly clonal cell line – that is, derived from a single cell.
For workflows requiring high numbers of clones, for example, multi-gene editing, this requirement for a clonal iPSC cell line represents a significant challenge.
iPSCs are sensitive and labour-intensive to culture. Traditional methods such as limiting dilution or colony picking yield low numbers of clones for downstream evaluation. With little or no documentation on the clonal origin of a cell line, this can become a real issue for researchers needing to satisfy regulatory requirements in the future.
At Cellected we can generate 100’s of single cell derived iPSC clones through our optimised platform, protocols and automated single cell seeding equipment. We can then use a variety of sophisticated assays to assess and triage the clones for those most suitable and of the highest quality, whilst at all times retaining the genetic integrity of the clones.
Provision of high-quality clonal iPSC lines
If you want a fully documented, clonal iPSC cell line – including images demonstrating clonality (which have been used in FDA submissions) – without the hassle, then contact Cellected.
We can generate a fully documented monoclonal cell line using specialist single cell seeding equipment. We use the VIPSTM from Advanced Instruments.
Once we have generated the clonal iPSC line, we will QC, validate and cryopreserve it and ship the clonal iPSC line to you. Hassle free. Rapid. Efficient. Documented. QC-ed.
